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earles basic salt solution  (Fisher Scientific)

 
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    Fisher Scientific earles basic salt solution
    Earles Basic Salt Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/earles basic salt solution/product/Fisher Scientific
    Average 90 stars, based on 1 article reviews
    earles basic salt solution - by Bioz Stars, 2026-03
    90/100 stars

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    C3 taken up by airway epithelial cells mitigates stress-induced cell death. (A–C) BEAS-2B cells were grown to confluence in a 12-well plate and treated with either serum-free media or H2O2 (500 μM) for 60 minutes, washed, and incubated with 10% C3 dp serum, 10% NHS, C3 dp serum + C3 (15 μg/ml), or C3 dp serum + C3-methylamine (C3-MA, 15 μg/ml) for 3 hours. The proportion of live cells (Q4) and dead cells (Q2 + Q3) was determined using annexin (x-axis) and propidium iodide (PI, y-axis) staining by flow cytometry. (A and B) Graphs represent the mean ± SEM of three to five replicates for each condition. (C) Representative flow-cytometry plots for each condition. (D–F) To evaluate whether the cytoprotective effect of C3 extends beyond H2O2-induced cell death, a growth factor deprivation model was used. BEAS-2B cells were grown to confluence in a 12-well plate and incubated with <t>Earle’s</t> balanced salt solution (EBSS) for 4 hours in the absence or presence of 10% C3 dp serum, 10% NHS, or C3 (15 μg/ml). (D and E) Graphs represent the mean ± SEM of two or three replicates for each condition. (F) Representative flow-cytometry plots for each condition. *P < 0.05, **P < 0.01. Representative of three independent experiments.
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    C3 taken up by airway epithelial cells mitigates stress-induced cell death. (A–C) BEAS-2B cells were grown to confluence in a 12-well plate and treated with either serum-free media or H2O2 (500 μM) for 60 minutes, washed, and incubated with 10% C3 dp serum, 10% NHS, C3 dp serum + C3 (15 μg/ml), or C3 dp serum + C3-methylamine (C3-MA, 15 μg/ml) for 3 hours. The proportion of live cells (Q4) and dead cells (Q2 + Q3) was determined using annexin (x-axis) and propidium iodide (PI, y-axis) staining by flow cytometry. (A and B) Graphs represent the mean ± SEM of three to five replicates for each condition. (C) Representative flow-cytometry plots for each condition. (D–F) To evaluate whether the cytoprotective effect of C3 extends beyond H2O2-induced cell death, a growth factor deprivation model was used. BEAS-2B cells were grown to confluence in a 12-well plate and incubated with Earle’s balanced salt solution (EBSS) for 4 hours in the absence or presence of 10% C3 dp serum, 10% NHS, or C3 (15 μg/ml). (D and E) Graphs represent the mean ± SEM of two or three replicates for each condition. (F) Representative flow-cytometry plots for each condition. *P < 0.05, **P < 0.01. Representative of three independent experiments.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Intracellular C3 Protects Human Airway Epithelial Cells from Stress-associated Cell Death

    doi: 10.1165/rcmb.2017-0405OC

    Figure Lengend Snippet: C3 taken up by airway epithelial cells mitigates stress-induced cell death. (A–C) BEAS-2B cells were grown to confluence in a 12-well plate and treated with either serum-free media or H2O2 (500 μM) for 60 minutes, washed, and incubated with 10% C3 dp serum, 10% NHS, C3 dp serum + C3 (15 μg/ml), or C3 dp serum + C3-methylamine (C3-MA, 15 μg/ml) for 3 hours. The proportion of live cells (Q4) and dead cells (Q2 + Q3) was determined using annexin (x-axis) and propidium iodide (PI, y-axis) staining by flow cytometry. (A and B) Graphs represent the mean ± SEM of three to five replicates for each condition. (C) Representative flow-cytometry plots for each condition. (D–F) To evaluate whether the cytoprotective effect of C3 extends beyond H2O2-induced cell death, a growth factor deprivation model was used. BEAS-2B cells were grown to confluence in a 12-well plate and incubated with Earle’s balanced salt solution (EBSS) for 4 hours in the absence or presence of 10% C3 dp serum, 10% NHS, or C3 (15 μg/ml). (D and E) Graphs represent the mean ± SEM of two or three replicates for each condition. (F) Representative flow-cytometry plots for each condition. *P < 0.05, **P < 0.01. Representative of three independent experiments.

    Article Snippet: The cells were also treated with serum-starved media, Earle’s basic salt solution (EBSS; no calcium, magnesium, or phenol red; Thermo Fisher Scientific), or 250 nM staurosporine (Sigma-Aldrich) for 4 hours in the presence or absence of an exogenous source of C3, as detailed above.

    Techniques: Incubation, Staining, Flow Cytometry